High Z resolution of a 3D image

High Z resolution

The Z resolution of a 3D image is defined by the number of scans in the z axis and the size of the Light Sheet. The number of scans can be easily increased to achieve higher resolution but the size of the light sheet is a characteristic of the system that cannot be changed. In all the configurations with illumination from different angles, the size of the Light sheet is rather big (from 2 to 24 µm depending on manufacturer and number of illumination angles). The QLS-scope has a Light Sheet of around 1 µm so it can provide the highest z resolution in the market.





TetraSpec fluorescent beads (Invitrogen) of 4um in 2% agarose with 2x objective. a), b) and c) are zoomed images while d) is the complete image.