Refractive index matching

Refractive index matching

In microscopy, the path light travels to and from the sample determines the quality of the final image at several levels: if the excitation beam encounters dense spots or translucent areas, the intensity of the illumination will decrease and its focus may change. If fluorescence from excited fluorophores in the sample goes through media with heterogeneous refractive indexes (RI), the light will suffer refractive changes at each interface, degrading the image and decreasing resolution.

To avoid these deleterious effects, modifications are incorporated in the optics (i.e. RI correction collars) which increase the cost and complexity of the devices. QLS-Scope solves this issue by employing a set of especially designed imaging glassware and media that present a homogenous refractive index, irrespective of the clearing protocol used on the sample. They include tubes of different materials and diameters to accommodate any sample, and their cylindrical section allows to rotate the sample to optimize positioning, as well as the application of SPIM-SPOT.

Please see more details on the related application note (link al application note)