Technology QLS
QLS-Clear
Our QLS-Clear is designed for automated optical clearing and immunostaining of large biological tissues, from millimeter-scale biopsies to entire mouse organs. By combining controlled reagent exchange, precise temperature and agitation, and software-driven protocols, the system delivers highly reproducible, hands-off clearing and immunolabeling — ready for light-sheet, confocal, or multiphoton microscopy.
Why Automation Matters
- Eliminates batch-to-batch variability
- Prevents sample damage during manual handling
- Reduces hands-on time of qualified personnel
Ensures reproducible labeling across experiments
Key Features
- Different large tissues can be processed (e.g. whole organs, embryos, etc.) with different methods
- Additional processing steps apart of the clearing (e.g. bleaching, decalcification, immunolabeling) can be integrated and performed automatically
- Up to three immunolabeling steps can be integrated for the same sample.
- Be able to introduce a new sample while the system is running with completely different processing parameters than the samples running
Specifications
| Cooling Module | |
| Temperature Range | 4-6°C |
| Large capacity | 20L (available space for sample storage) |
| Number of Reagents |
Up to 11 · 1 Buffer · 2 Clearing agents · 3 immunolabeling methods (6 reagents) · 1 Bleaching reagent · 1 Decalcification reagent |
| Temperature Stability | ±0.2°C |
| Incubation Module | |
| Temperature Range | Ambient +5 to 70°C (Increments of 0.1°C). |
| Temperature Uniformity | ±0.5 °C |
| Temperature Accuracy | ±0.1 °C |
| Shaking Speed Range | 30 to 300 rpm (Increments of 1 rpm) |
| Shaking Orbit | 19 mm (3/4″) |
| Sample Module | |
| Number of samples | 5, 7 or 11 (others upon request) |
| Sample size | Up to 15 mm (d) x 15mm (h) (others upon request) |
| Software | |
| User access control | |
| Run each sample independently at any time | |
| Different methods can be used in each sample | |
| Step time in seconds, minutes, hours or days | |
| Zero loss of reagent | |
| Reagents and waste volume control | |
| Operation conditions | |
| Permissible ambient temperature | < 35 oC, 27 oC optimum |
| Permissible relative air humidity (no condens.) | < 65 % at 30 °C |
| Size | 60 x 45 x 30 cm |
| Weight | 70 kg for imaging unit, 5 kg for power supply/PC |
