Technology QLS
QLS-scope LS
Imaging large biological tissues requires a fundamentally different optical approach than conventional high-magnification microscopy. Our QLS-Scope LS is specifically designed to handle very large samples. By combining a low magnification air objective and a zoom lens it can achieve extended fields of view while keeping a long working distance, enabling intact imaging of whole organs and large cleared specimens without sectioning.
Key Features
- Sample rotation
- Motorized XYZ stage
- Double side illumination through 2X objectives
- White light Illumination for sample viewing and OPT mode
- SPIM and multi-angle SPIM
- SPOT measuring mode for high axial resolution
- 4 laser lines
Specifications
| Optics | |
| Light Sheet thickness | approx. 1 µm |
| Magnification | 0.66X – 4.64X |
| Numerical aperture | 0.049 – 0.142 |
| Field of view (mm) | 16.67 – 2.37 |
| Resolving power (µm) | 6.81 – 2.35 |
| Illumination | |
| Wavelength |
405, 488, 561, 640 nm Other wavelengths are also available upon request. |
| Power | Up to 30-65 mW, depending on the wavelength |
| Detection | |
| Type | sCMOS |
| Pixel | 3000 x 3000 |
| Sensor size | 11.280 x 11.280 mm |
| Pixel size | 3.76 µm |
| Maximum frame rate | 18 fps at full resolution |
| Read noise | < 3.4 e– at full resolution |
| Cooling | 10oC with air cooling |
| Imaging chamber | |
| Sample travel range (X, Y, Z) |
XYZ: 50 mm, 50 mm, 50 mm Step resolution 0.1 µm |
| Sample rotation |
Up to 360o Accuracy 0.14o |
| Sample size | 0.1 mm to 40 mm in diameter, 40 mm in length |
| Operation conditions | |
| Permissible ambient temperature | < 35 oC, 27 oC optimum |
| Permissible relative air humidity (no condens.) | < 65 % at 30 °C |
| Size | 60 x 45 x 30 cm |
| Weight | 70 kg for imaging unit, 5 kg for power supply/PC |
Applications
Whole-Organ & Large-Tissue Imaging (Cleared Samples)
- Whole-organ cell mapping
- 3D cytoarchitecture reconstruction
- Long-range connectivity analysis (e.g. axonal tracts)
- Organ-wide gene or protein expression mapping
Pathology, Histology & 3D Diagnostics (Research Use)
- 3D histopathology (R&D / pre-clinical)
- Tumor margin visualization
- Vascular infiltration studies
- Fibrosis and tissue remodeling analysis
Developmental Biology & Organogenesis
- Embryo development (mid–late stages)
- Organ growth dynamics (fixed timepoints)
- Morphogenesis defects
- Genetic perturbation studies
Cancer Research & Tumor Biology
- 3D tumor microenvironment analysis
- Immune-cell infiltration
- Metastatic niche mapping
- Drug penetration studies
Immunology & Cell-Migration Studies
- Immune-cell spatial distribution
- Lymph node architecture
- Inflammation models
- Host–pathogen interactions
Tissue Engineering & Regenerative Medicine
- Thick organoids and assembloids
- Bioprinted tissues
- Cell-scaffold integration
- Vascularization of engineered constructs
Plant Biology & Large Botanical Samples
- Root system architecture
- Vascular transport studies
- Developmental gradients
- Stress and pathogen response