Technology QLS
QLS-scope LV
For live 3D imaging, acquisition speed is critical. Biological dynamics occur on short time scales, and slow volumetric scanning can lead to motion artifacts, reduced temporal resolution, and increased phototoxicity.
Our QLS-Scope LV is designed for high-speed, coordinated scanning, where the light sheet and the detection focal plane move synchronously through the sample in 10ms per step. This optomechanical approach enables rapid volumetric acquisition without repeated sample repositioning, preserving spatial accuracy while dramatically reducing imaging time.
By minimizing mechanical overhead and maximizing optical efficiency, the system captures 3D volumes fast enough to follow dynamic biological processes in living samples. The result is high-temporal-resolution light-sheet imaging with reduced light exposure, making it ideally suited for long-term, gentle observation of live specimens.
Key Features
- Sample rotation
- Motorized XYZ stage
- Double side illumination through 2X objectives
- White light Illumination for sample viewing
- Fast SPIM
- 4 laser lines
- Incubator integration
Specifications
|
Optics |
|
|
Light Sheet thickness |
approx. 1 µm |
|
Magnification |
6.40X – 45.00X |
|
Numerical aperture |
0.468 – 0.600 |
|
Field of view (mm) |
1.20 – 0.36 |
|
Resolving power (µm) |
0.72 – 0.56 |
|
Illumination |
|
|
Wavelength |
405, 488, 561, 640 nm Other wavelengths are also available upon request. |
|
Power |
Up to 30-65 mW, depending on the wavelength |
|
Detection |
|
|
Type |
sCMOS |
|
Pixel |
2048×2028 |
|
Sensor size |
13.3 x 13.3 mm |
|
Pixel size |
6.5 µm |
|
Maximum frame rate |
100 fps at full resolution |
|
Read noise |
< 1 e– at full resolution |
|
Cooling |
– 10oC with air cooling |
|
Imaging chamber |
|
|
Sample travel range (X, Y, Z) |
XYZ: 10 mm, 10 mm, 10 mm Step resolution 0.1 µm |
|
Sample rotation |
Up to 360o Accuracy 0.14o |
|
Sample size |
0.1 mm to 3 mm in diameter, 3 mm in length |
|
Incubation |
|
|
Temperature Stability |
0.1 degrees |
|
CO2-Module |
Available |
|
Humidity control |
Available |
|
Temperature range |
From room temperature to 50oC |
|
Operation conditions |
|
|
Permissible ambient temperature |
< 35 oC, 27 oC optimum |
|
Permissible relative air humidity (no condens.) |
< 65 % at 30 °C |
|
Size |
60 x 45 x 30 cm |
|
Weight |
70 kg for imaging unit, 5 kg for power supply/PC |
Applications
Whole-Brain Functional Imaging
- Whole-brain calcium imaging
- Neuronal population dynamics
- Sensory processing & behavior coupling
- Brain-wide activity maps at kHz rates
Fast Neural Dynamics & Circuit Physiology
- Action-potential–related calcium transients
- Network synchronization
- Functional connectivity mapping
- Neuromodulation experiments (optogenetics + LSFM)
Organoids, Spheroids & 3D Cell Models (Live)
- Developmental dynamics
- Cell–cell interaction studies
- Drug response kinetics
- Functional heterogeneity mapping
Cardiac & Muscle Physiology (Beating Samples)
- Beating heart imaging (zebrafish, embryos)
- Calcium wave propagation
- Electromechanical coupling
- Drug cardiotoxicity screening
Fast Developmental Biology & Morphodynamics
- Cell migration and rearrangement
- Tissue folding and morphogenesis
- Symmetry breaking
- Early embryogenesis (fish, insects)
Host–Pathogen & Infection Dynamics
- Immune cell trafficking
- Pathogen spread in 3D tissues
- Inflammation dynamics
- Antibiotic or antiviral response
High-Throughput Functional Phenotyping
- Genetic screening (zebrafish, flies)
- Drug screening with functional readouts
- Toxicology
- Behavioral–neural correlation studies
udies
- Immune-cell spatial distribution
- Lymph node architecture
- Inflammation models
- Host–pathogen interactions
Tissue Engineering & Regenerative Medicine
- Thick organoids and assembloids
- Bioprinted tissues
- Cell-scaffold integration
- Vascularization of engineered constructs
Plant Biology & Large Botanical Samples
- Root system architecture
- Vascular transport studies
- Developmental gradients
- Stress and pathogen response