Technology QLS

Light-sheet fluorescence microscopy provides fast, high-resolution imaging of labeled structures, but like all fluorescence techniques, it cannot visualize unlabeled anatomy. As a result, fluorescent signals often appear without structural context, making it difficult to interpret tissue organization, growth, or migration within the surrounding sample.

Optical Projection Tomography (OPT) addresses this limitation by imaging the intrinsic optical contrast of the specimen using transmitted white light. By acquiring hundreds of projections from different angles and reconstructing them tomographically, OPT delivers a complete 3D representation of the sample’s anatomy without the need for labels or high laser power.

Our unique platform combines white light OPT for structural imaging with SPIM fluorescence for molecular specificity, producing perfectly co-registered 3D datasets. This enables researchers to visualize fluorescent features within their true anatomical environment, as demonstrated by whole-organ reconstructions such as mouse lung, where labeled structures are seen in full anatomical context